HIF2α/EPAS1 Recombinant antibody, PBS Only (Detector)
HIF2α/EPAS1 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
WB, Cytometric bead array, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
240736F2
Cat no : 83790-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
83790-1-PBS targets HIF2α/EPAS1 as part of a matched antibody pair:
MP00775-1: 83790-4-PBS capture and 83790-1-PBS detection (validated in Cytometric bead array)
MP00775-2: 83790-2-PBS capture and 83790-1-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | HIF2α/EPAS1 fusion protein Ag15199 相同性解析による交差性が予測される生物種 |
| Full Name | endothelial PAS domain protein 1 |
| Calculated molecular weight | 96 kDa |
| Observed molecular weight | 100-120 kDa |
| GenBank accession number | BC051338 |
| Gene symbol | HIF2α |
| Gene ID (NCBI) | 2034 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
HIF2A, also named as EPAS1, is a 870 amino acid protein, which is expressed in most tissues, with highest levels in placenta, lung and heart. HIF2A colocalizes with HIF3A in the nucleus and speckles. HIF2A as a transcription factor involves in the induction of oxygen regulated genes. HIF2A binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. HIF2A regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. HIF2A may also play a role in the formation of the endothelium that gives rise to the blood brain barrier. The calculated molecular weight of HIF2A is 96 kDa, but In normoxia, HIF2A is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. The modified HIf2A is about 100-120 kDa.
