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Host
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIF2α/EPAS1 Recombinant antibody, PBS Only (Capture)

HIF2α/EPAS1 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240736H7

Cat no : 83790-4-PBS

Synonyms

EPAS1, Endothelial PAS domain-containing protein 1, ECYT4, Class E basic helix-loop-helix protein 73, bHLHe73

Validation Data Gallery

Filter:
  • Sandwich ELISA standard curve of MP00775-4

    Sandwich ELISA standard curve of MP00775-4

    Sandwich ELISA standard curve of MP00775-4, Human EPAS1 Recombinant Matched Antibody Pair - PBS only. 83790-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15199. 83790-5-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

  • Sample test of MP00775-4

    Sample test of MP00775-4

    A549 and Hela cell lysates were measured. The human EPAS1 concentration of detected samples was determined to be 7,062.3 pg/mL (based on a 1.00 mg/mL extract load) in A549 cell lysate and 6,988.6 pg/mL (based on a 1.50 mg/mL extract load) in Hela cell lysate.

  • Cytometric bead array standard curve of MP00775-1

    Cytometric bead array standard curve of MP00775-1

    Cytometric bead array standard curve of MP00775-1, HIF2α/EPAS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83790-4-PBS. Detection antibody: 83790-1-PBS. Standard: Ag15199. Range: 0.78-100 ng/mL.

  • Cytometric bead array standard curve of MP00775-3

    Cytometric bead array standard curve of MP00775-3

    Cytometric bead array standard curve of MP00775-3, HIF2α/EPAS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83790-4-PBS. Detection antibody: 83790-3-PBS. Standard: Ag15199. Range: 1.56-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83790-4-PBS targets HIF2α/EPAS1 as part of a matched antibody pair:

MP00775-1: 83790-4-PBS capture and 83790-1-PBS detection (validated in Cytometric bead array)

MP00775-3: 83790-4-PBS capture and 83790-3-PBS detection (validated in Cytometric bead array)

MP00775-4: 83790-4-PBS capture and 83790-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HIF2α/EPAS1 fusion protein Ag15199 相同性解析による交差性が予測される生物種
Full Name endothelial PAS domain protein 1
Calculated molecular weight 96 kDa
GenBank accession numberBC051338
Gene symbol HIF2α
Gene ID (NCBI) 2034
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.