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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EPB41L3 Polyclonal antibody

EPB41L3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10719-1-AP

Synonyms

Erythrocyte membrane protein band 4.1-like 3, DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using EPB41L3 Polyclonal antibody (10719-1-AP)

    WB analysis using 10719-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10719-1-AP (EPB41L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using EPB41L3 Polyclonal antibody (10719-1-AP)

    IHC staining of human brain using 10719-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10719-1-AP (EPB41L3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using EPB41L3 Polyclonal antibody (10719-1-AP)

    IHC staining of human brain using 10719-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10719-1-AP (EPB41L3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inPC-3 cells, U2OS cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10719-1-AP targets EPB41L3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPB41L3 fusion protein Ag1089 相同性解析による交差性が予測される生物種
Full Name erythrocyte membrane protein band 4.1-like 3
Calculated molecular weight 121 kDa
Observed molecular weight120-130 kDa
GenBank accession numberBC008377
Gene symbol EPB41L3
Gene ID (NCBI) 23136
RRIDAB_2098488
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EPB41L3, also known as 4.1B and DAL1, belongs to the protein 4.1 family. It is a tumor suppressor that inhibits cell proliferation and promotes apoptosis. EPB41L3 modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5 (PMID: 15334060, 15737618). DAL1 loss is an early event in meningioma tumorigenesis (PMID: 10888600).

Protocols

Product Specific Protocols
WB protocol for EPB41L3 antibody 10719-1-APDownload protocol
IHC protocol for EPB41L3 antibody 10719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

The Role of Erythrocyte Membrane Protein Band 4.1-like 3 in Idiopathic Pulmonary Fibrosis

Authors - Min Kyung Kim
View Article