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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPB41L4B Recombinant antibody (84732-2-RR)

    WB analysis using 84732-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84732-2-RR (EPB41L4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84732-2-RR

    Affinity and Kinetic Characterization of 84732-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84732-2-RR against Human EPB41L4B were performed. The affinity constant is 6.86 nM.

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EPB41L4B Recombinant antibody

EPB41L4B Recombinant Antibody for WB, ELISA
Cat No. 84732-2-RR
Clone No.242186G4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

FERM containing protein CG1, EHM2, CG1, Band 4.1 like protein 4B, 242186G4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293T cells, HepG2 cells, HaCaT cells, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84732-2-RR targets EPB41L4B in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EPB41L4B fusion protein Ag36353 相同性解析による交差性が予測される生物種
Full Name erythrocyte membrane protein band 4.1 like 4B
Calculated molecular weight99kDa
Observed molecular weight100 kDa
GenBank accession numberNM_00138562
Gene Symbol EPB41L4B
Gene ID (NCBI) 54566
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H329
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 4.1 proteins, encoded by the EPB41 (erythrocyte protein band 4.1) genes, are components of the cortical cytoskeleton underlying the cell membrane. The family of 4.1 proteins consists of the eponymous 4.1R protein first identified in erythrocytes (gene: EPB41), 4.1N (EPB41L1), 4.1G (EPB41L2), 4.1B (EPB41L3) as well as the less closely related members NBL4 (EPB41L4A), EHM2 (EPB41L4B) and EPB41L5 (EPB41L5). EHM2 is conspicuous in tumor cells with high migratory potential, such as metastatic melanoma and fibrosarcoma cells. In prostate cancer, EHM2 has been reported to be overexpressed and to diminish adhesion of prostate cancer cells to collagen. (PMID: 20860828)

Protocols

Product Specific Protocols
WB protocol for EPB41L4B antibody 84732-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols