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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPB42 Polyclonal antibody (27443-1-AP)

    WB analysis using 27443-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27443-1-AP (EPB42 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EPB42 Polyclonal antibody (27443-1-AP)

    WB analysis using 27443-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27443-1-AP (EPB42 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using EPB42 Polyclonal antibody (27443-1-AP)

    IHC staining of human placenta using 27443-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27443-1-AP (EPB42 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using EPB42 Polyclonal antibody (27443-1-AP)

    IHC staining of human placenta using 27443-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27443-1-AP (EPB42 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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EPB42 Polyclonal antibody

EPB42 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27443-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SPH5, Protein 4.2, PA, P4.2, Erythrocyte protein 4.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27443-1-AP targets EPB42 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPB42 fusion protein Ag23556 相同性解析による交差性が予測される生物種
Full Name erythrocyte membrane protein band 4.2
Observed molecular weight72 kDa
GenBank accession numberBC096093
Gene Symbol EPB42
Gene ID (NCBI) 2038
RRIDAB_3085962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16452
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EPB42, also known as Protein 4.2, is amongst the most abundant protein components of the erythrocyte membrane, representing approximately 5% membrane content or ~2.5 × 105 copies/cell. EPB42 binds to the cytoplasmic domain of band 3, which serves as a site for the anchorage of several membrane skeletal proteins(PMID: 19269200, 12049649). EPB42 is a 72 kDa peripheral membrane protein.

Protocols

Product Specific Protocols
WB protocol for EPB42 antibody 27443-1-APDownload protocol
IHC protocol for EPB42 antibody 27443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols