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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using EPB42 Recombinant antibody (84921-3-RR)

    WB analysis of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84921-3-RR (EPB42 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using EPB42 Recombinant antibody (84921-3-RR)

    IHC staining of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84921-3-RR (EPB42 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using EPB42 Recombinant antibody (84921-3-RR)

    IHC staining of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84921-3-RR (EPB42 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEL cells using EPB42 Recombinant antibody (84921-3-RR)

    IF Staining of HEL using 84921-3-RR (same clone as 84921-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEL cells using EPB42 antibody (84921-3-RR, Clone: 242412F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01708-1

    Cytometric bead array standard curve of MP01708-1

    Cytometric bead array standard curve of MP01708-1, EPB42 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84921-2-PBS. Detection antibody: 84921-3-PBS. Standard: Ag23556. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01708-2

    Cytometric bead array standard curve of MP01708-2

    Cytometric bead array standard curve of MP01708-2, EPB42 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84921-1-PBS. Detection antibody: 84921-3-PBS. Standard: Ag23556. Range: 1.25-80 ng/mL.

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EPB42 Recombinant antibody, PBS Only (Detector)

EPB42 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84921-3-PBS
Clone No.242412F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

242412F4, E42P, Erythrocyte protein 4.2, P4.2, PA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84921-3-PBS targets EPB42 as part of a matched antibody pair:

MP01708-1: 84921-2-PBS capture and 84921-3-PBS detection (validated in Cytometric bead array)

MP01708-2: 84921-1-PBS capture and 84921-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EPB42 fusion protein Ag23556 相同性解析による交差性が予測される生物種
Full Name erythrocyte membrane protein band 4.2
Observed molecular weight77 kDa
GenBank accession numberBC096093
Gene Symbol EPB42
Gene ID (NCBI) 2038
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16452
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

EPB42, also known as Protein 4.2, is amongst the most abundant protein components of the erythrocyte membrane, representing approximately 5% membrane content or ~2.5 × 105 copies/cell. EPB42 binds to the cytoplasmic domain of band 3, which serves as a site for the anchorage of several membrane skeletal proteins(PMID: 19269200, 12049649). EPB42 is a 72 kDa peripheral membrane protein.