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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EPCAM/CD326 Recombinant antibody, PBS Only

EPCAM/CD326 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241243B10

Cat no : 84073-6-PBS

Synonyms

EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPCAM/CD326 Recombinant antibody (84073-6-RR)

    WB analysis using 84073-6-RR (same clone as 84073-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using EPCAM/CD326 Recombinant antibody (84073-6-RR)

    IHC staining of human ovary cancer using 84073-6-RR (same clone as 84073-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using EPCAM/CD326 Recombinant antibody (84073-6-RR)

    IHC staining of human ovary cancer using 84073-6-RR (same clone as 84073-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using EPCAM/CD326 Recombinant antibody (84073-6-RR)

    IF Staining of A431 using 84073-6-RR (same clone as 84073-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using EPCAM/CD326 antibody (84073-6-RR, Clone: 241243B10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84073-6-PBS is EPCAM/CD326 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name epithelial cell adhesion molecule
Calculated molecular weight35 kDa
Observed molecular weight35-40 kDa
GenBank accession numberNM_002354.3
Gene symbol EPCAM
Gene ID (NCBI) 4072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)