Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
27252-1-AP targets EPDR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EPDR1 fusion protein Ag26120 相同性解析による交差性が予測される生物種 |
| Full Name | Mammalian ependymin-related protein 1 |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 25-30 kDa |
| GenBank accession number | BC018299 |
| Gene Symbol | EPDR1 |
| Gene ID (NCBI) | 54749 |
| RRID | AB_3085942 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UM22 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ependymin-related 1 (EPDR1) gene, also known as MERP1 and UCC1, encodes for a protein related to ependymins, which are type II transmembrane proteins that are similar to two families of cell adhesion molecules: the protocadherins and ependymins. Ependymin-related protein 1 (EPDR1) was recently identified as a secreted human batokine regulating mitochondrial respiration linked to thermogenesis in brown fat. Proteomics studies of mammalian mannose-6-phosphate (M6P) glycoproteins have identified EPDR1 as a lysosomal protein with unknown function. The lysosomal localization of EPDR1 was further demonstrated in mouse brain homogenates by subcellular fractionation.(PMID: 36343918, PMID: 33902536, PMID: 30729188)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EPDR1 antibody 27252-1-AP | Download protocol |
| IHC protocol for EPDR1 antibody 27252-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Clin Exp Pathol Overexpression of EPDR1 has an antitumorigenic effect on breast cancer in vitro. | ||
Heliyon Identification of lysosome-related hub genes as potential biomarkers and immune infiltrations of moyamoya disease by multiple bioinformatics methods and machine-learning strategies |