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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using EPHA1-specific Monoclonal antibody (60155-2-Ig)

    WB analysis of HeLa using 60155-2-Ig (same clone as 60155-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using EPHA1-specific Monoclonal antibody (60155-2-Ig)

    WB analysis of COLO 320 using 60155-2-Ig (same clone as 60155-2-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using EPHA1-specific Monoclonal antibody (60155-2-Ig)

    WB analysis of HepG2 using 60155-2-Ig (same clone as 60155-2-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EPHA1-specific Monoclonal antibody (60155-2-Ig)

    IHC staining of human breast cancer using 60155-2-Ig (same clone as 60155-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EPHA1-specific Monoclonal antibody (60155-2-Ig)

    IHC staining of human breast cancer using 60155-2-Ig (same clone as 60155-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EPHA1-specific Monoclonal antibody (60155-2-Ig)

    IHC staining of human stomach cancer using 60155-2-Ig (same clone as 60155-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EPHA1-specific Monoclonal antibody (60155-2-Ig)

    IHC staining of human stomach cancer using 60155-2-Ig (same clone as 60155-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using EPHA1-specific Monoclonal antibody (60155-2-Ig)

    IHC staining of human liver cancer using 60155-2-Ig (same clone as 60155-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using EPHA1-specific Monoclonal antibody (60155-2-Ig)

    IHC staining of human liver cancer using 60155-2-Ig (same clone as 60155-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

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EPHA1-specific Monoclonal antibody, PBS Only

EPHA1-specific Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60155-2-PBS
Clone No.1B7C3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

EPH, EPH receptor A1, EPHA1, EPHA1-specific, Ephrin type A receptor 1, EPHT, EPHT1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60155-2-PBS targets EPHA1-specific in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name EPH receptor A1
Calculated molecular weight 108 kDa
Observed molecular weight 108 kDa
GenBank accession numberBC130291
Gene Symbol EPHA1
Gene ID (NCBI) 2041
RRIDAB_10646478
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP21709
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

EPHA1, also named as EPH, EPHT and EPHT1, belongs to ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The general role of the Eph family is in mediating repulsive cell-cell interaction.