Validation Data Gallery
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Tested Applications
| Positive WB detected in | A375 cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
25749-1-AP targets EPHA2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EPHA2 fusion protein Ag22661 相同性解析による交差性が予測される生物種 |
| Full Name | EPH receptor A2 |
| Calculated molecular weight | 976 aa, 108 kDa |
| Observed molecular weight | 125-130 kDa |
| GenBank accession number | BC037166 |
| Gene Symbol | EPHA2 |
| Gene ID (NCBI) | 1969 |
| RRID | AB_3669497 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29317 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EPHA2 (Ephrin type-A receptor 2) belongs to the receptor tyrosine kinase (RTK) family, with 16 known receptors and 9 known membrane-bound ligands in all species. Based on the extracellular domain sequence homology, structure, and binding affinity, Eph receptors and their ephrin ligands can be divided into A and B subtypes (PMID: 33962882). EPHA2 contains a conserved N-terminal ligand-bound extracellular domain, a transmembrane domain, and a conserved tyrosine kinase domain (PMID: 8070404).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EPHA2 antibody 25749-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Anticancer Res High αv Integrin Level of Cancer Cells Is Associated with Development of Brain Metastasis in Athymic Rats. |