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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPHA2 Monoclonal antibody (66736-1-Ig)

    WB analysis using 66736-1-Ig (same clone as 66736-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66736-1-Ig (EPHA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66736-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EPHA2 Monoclonal antibody (66736-1-Ig)

    IF Staining of HepG2 using 66736-1-Ig (same clone as 66736-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPHA2 antibody (66736-1-Ig, Clone: 1A9C3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66736-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

EPHA2 Monoclonal antibody, PBS Only

EPHA2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66736-1-PBS
Clone No.1A9C3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Ephrin type-A receptor 2, Ephrin type A receptor 2, EPH receptor A2, ECK, EC:2.7.10.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66736-1-PBS targets EPHA2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EPHA2 fusion protein Ag22566 相同性解析による交差性が予測される生物種
Full Name EPH receptor A2
Calculated molecular weight 976 aa, 108 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC037166
Gene Symbol EPHA2
Gene ID (NCBI) 1969
RRIDAB_2882086
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29317
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

EPHA2 (Ephrin type-A receptor 2) belongs to the receptor tyrosine kinase (RTK) family, with 16 known receptors and 9 known membrane-bound ligands in all species. Based on the extracellular domain sequence homology, structure, and binding affinity, Eph receptors and their ephrin ligands can be divided into A and B subtypes (PMID: 33962882). EPHA2 contains a conserved N-terminal ligand-bound extracellular domain, a transmembrane domain, and a conserved tyrosine kinase domain (PMID: 8070404).