• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EPHA5 Polyclonal antibody

EPHA5 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55215-1-AP

Synonyms

CEK7, EHK 1, EHK1, EK7, EPH homology kinase 1, EPH like kinase 7, EPH receptor A5, EPHA5, Ephrin type A receptor 5, HEK7, TYRO4

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse brain tissue using EPHA5 Polyclonal antibody (55215-1-AP)

    IHC staining of mouse brain using 55215-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55215-1-AP (EPHA5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EPHA5 Polyclonal antibody (55215-1-AP)

    IHC staining of mouse brain using 55215-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55215-1-AP (EPHA5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using EPHA5 Polyclonal antibody (55215-1-AP)

    IF Staining of SH-SY5Y using 55215-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 55215-1-AP (EPHA5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55215-1-AP targets EPHA5 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name EPH receptor A5
Calculated molecular weight 115 kDa
GenBank accession numberNM_004439
Gene symbol EPHA5
Gene ID (NCBI) 2044
RRIDAB_2881289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for EPHA5 antibody 55215-1-APDownload protocol
IF protocol for EPHA5 antibody 55215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols