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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EPHA7 Monoclonal antibody, PBS Only

EPHA7 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A7E5

Cat no : 66667-1-PBS

Synonyms

EHK 3, EHK3, EK11, EPH homology kinase 3, EPH like kinase 11, EPH receptor A7, EPHA7, Ephrin type A receptor 7, HEK11

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using EPHA7 Monoclonal antibody (66667-1-Ig)

    WB analysis of human brain using 66667-1-Ig (same clone as 66667-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66667-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using EPHA7 Monoclonal antibody (66667-1-Ig)

    WB analysis of pig brain using 66667-1-Ig (same clone as 66667-1-PBS)

    Untreated and PNGase F-treated lysates of pig brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66667-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using EPHA7 Monoclonal antibody (66667-1-Ig)

    WB analysis of rat brain using 66667-1-Ig (same clone as 66667-1-PBS)

    Untreated and PNGase F-treated lysates of rat brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66667-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using EPHA7 Monoclonal antibody (66667-1-Ig)

    WB analysis of mouse cerebellum using 66667-1-Ig (same clone as 66667-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66667-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using EPHA7 Monoclonal antibody (66667-1-Ig)

    WB analysis of pig brain using 66667-1-Ig (same clone as 66667-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66667-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using EPHA7 Monoclonal antibody (66667-1-Ig)

    WB analysis of rat brain using 66667-1-Ig (same clone as 66667-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66667-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66667-1-PBS targets EPHA7 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EPHA7 fusion protein Ag3813 相同性解析による交差性が予測される生物種
Full Name EPH receptor A7
Calculated molecular weight 998 aa, 112 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC027940
Gene symbol EPHA7
Gene ID (NCBI) 2045
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ephrin receptor A7 (EphA7) is a member of the Eph receptor family. EPHA7 plays a role in corticogenesis processes, determines brain size and shape, and is involved in development of the central nervous system (PMID:34176129). In addition, EphA7 also has a dual role of tumor promoter and tumor suppressor. It can participate in cell proliferation, migration and apoptosis through various mechanisms, and afect tumor diferentiation, staging and prognosis. EphA7 may be a potential diagnostic marker and tumor treatment target (PMID:35112312).