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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using EPHB1 Monoclonal antibody (67080-1-Ig)

    WB analysis of mouse cerebellum using 67080-1-Ig (same clone as 67080-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67080-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using EPHB1 Monoclonal antibody (67080-1-Ig)

    WB analysis of fetal human brain using 67080-1-Ig (same clone as 67080-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67080-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using EPHB1 Monoclonal antibody (67080-1-Ig)

    WB analysis of mouse brain using 67080-1-Ig (same clone as 67080-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67080-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50740-1

    Cytometric bead array standard curve of MP50740-1

    Cytometric bead array standard curve of MP50740-1, EPHB1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67080-2-PBS. Detection antibody: 67080-1-PBS. Standard:Ag16347. Range: 0.195-200 ng/mL.

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EPHB1 Monoclonal antibody, PBS Only (Detector)

EPHB1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 67080-1-PBS
Clone No.1B7C12

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

EPH receptor B1, EPH like kinase 6, ELK, EK6, EC:2.7.10.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67080-1-PBS targets EPHB1 as part of a matched antibody pair:

MP50740-1: 67080-2-PBS capture and 67080-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EPHB1 fusion protein Ag16347 相同性解析による交差性が予測される生物種
Full Name EPH receptor B1
Calculated molecular weight 984 aa, 110 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC111744
Gene Symbol EPHB1
Gene ID (NCBI) 2047
RRIDAB_2882388
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54762
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

EPHB1, also named as EPHT2, NET, HEK6 and ELK, belongs to the protein kinase superfamily, Tyr protein kinase family, and Ephrin receptor subfamily. It is a receptor for members of the ephrin-B family. EPHB1 binds to ephrin-B1, -B2 and -B3. EPHB1 may be involved in cell-cell interactions in the nervous system. EPHB1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.