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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPHX1 Monoclonal antibody (66982-1-Ig)

    WB analysis using 66982-1-Ig (same clone as 66982-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EPHX1 Monoclonal antibody (66982-1-Ig)

    WB analysis of HeLa using 66982-1-Ig (same clone as 66982-1-PBS)

    WB result of EPHX1 antibody (66982-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX1 transfected HeLa cells. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using EPHX1 Monoclonal antibody (66982-1-Ig)

    WB analysis of A549 using 66982-1-Ig (same clone as 66982-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using EPHX1 Monoclonal antibody (66982-1-Ig)

    WB analysis of 4T1 using 66982-1-Ig (same clone as 66982-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

EPHX1 Monoclonal antibody, PBS Only

EPHX1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66982-1-PBS
Clone No.1B6A2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB, Indirect ELISA

Synonyms

EPHX, EPHX1, EPOX, Epoxide hydratase, Epoxide hydrolase 1, MEH, Microsomal epoxide hydrolase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66982-1-PBS targets EPHX1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EPHX1 fusion protein Ag25820 相同性解析による交差性が予測される生物種
Full Name epoxide hydrolase 1, microsomal (xenobiotic)
Calculated molecular weight 53 kDa
Observed molecular weight53 kDa
GenBank accession numberBC003567
Gene Symbol EPHX1
Gene ID (NCBI) 2052
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07099
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EPHX1, also known as EPHX and EPOX, belongs to the peptidase S33 family. EPHX1 is an evolutionarily highly conserved biotransformation enzyme expressed in nearly all tissues and localized mainly in the microsomal fraction of the endoplasmic reticulum of eukaryotic cells. EPHX1 plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids. The calculated molecular weight of EPHX1 is 53 kDa (PMID: 26216302).