• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

EPLIN Polyclonal antibody, PBS Only

EPLIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16639-1-PBS

Synonyms

LIMA1, SREBP3, PP624, LIM domain and actin-binding protein 1, LIM domain and actin binding 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using EPLIN Polyclonal antibody (16639-1-AP)

    WB analysis of HEK-293 using 16639-1-AP (same clone as 16639-1-PBS)

    WB result of EPLIN antibody (16639-1-AP, 1:1000) with si-Control and si-EPLIN transfected HEK293 cells. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using EPLIN Polyclonal antibody (16639-1-AP)

    WB analysis of HUVEC using 16639-1-AP (same clone as 16639-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 16639-1-AP (EPLIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using EPLIN Polyclonal antibody (16639-1-AP)

    WB analysis of human placenta using 16639-1-AP (same clone as 16639-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16639-1-AP (EPLIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using EPLIN Polyclonal antibody (16639-1-AP)

    IP experiment of mouse liver using 16639-1-AP (same clone as 16639-1-PBS)

    IP result of anti-EPLIN (IP:16639-1-AP, 4ug; Detection:16639-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using EPLIN Polyclonal antibody (16639-1-AP)

    IHC staining of human prostate cancer using 16639-1-AP (same clone as 16639-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EPLIN Polyclonal antibody (16639-1-AP)

    IHC staining of human pancreas cancer using 16639-1-AP (same clone as 16639-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16639-1-AP (EPLIN antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using EPLIN Polyclonal antibody (16639-1-AP)

    IF Staining of PC-3 using 16639-1-AP (same clone as 16639-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using EPLIN antibody (16639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EPLIN Polyclonal antibody (16639-1-AP)

    IF Staining of HepG2 using 16639-1-AP (same clone as 16639-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPLIN antibody (16639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EPLIN Polyclonal antibody (16639-1-AP)

    IF Staining of HepG2 using 16639-1-AP (same clone as 16639-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using LIMA1 antibody 16639-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16639-1-PBS targets EPLIN in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPLIN fusion protein Ag9994 相同性解析による交差性が予測される生物種
Full Name LIM domain and actin binding 1
Calculated molecular weight 85 kDa
Observed molecular weight 90 kDa, 110 kDa
GenBank accession numberBC001247
Gene symbol EPLIN
Gene ID (NCBI) 51474
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues.