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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPN2 Recombinant antibody (84366-5-RR)

    WB analysis using 84366-5-RR (same clone as 84366-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84366-5-RR (EPN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84366-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using EPN2 Recombinant antibody (84366-5-RR)

    IHC staining of mouse lung using 84366-5-RR (same clone as 84366-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84366-5-RR (EPN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84366-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using EPN2 Recombinant antibody (84366-5-RR)

    IHC staining of mouse lung using 84366-5-RR (same clone as 84366-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84366-5-RR (EPN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84366-5-PBS in a different storage buffer formulation.

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EPN2 Recombinant antibody, PBS Only

EPN2 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84366-5-PBS
Clone No.241634F12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Epsin-2, epsin 2, EPS-15-interacting protein 2, EPS 15 interacting protein 2, EHB21

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84366-5-PBS targets EPN2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EPN2 fusion protein Ag35194 相同性解析による交差性が予測される生物種
Full Name epsin 2
Calculated molecular weight 584 aa, 62 kDa
Observed molecular weight62 kDa
GenBank accession numberBC093972
Gene Symbol EPN2
Gene ID (NCBI) 22905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO95208
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

EPN2 (EPS 15 interacting protein 2) is a protein that interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. EPN2 is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery.