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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPN3 Polyclonal antibody (20178-1-AP)

    WB analysis using 20178-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20178-1-AP (EPN3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EPN3 Polyclonal antibody (20178-1-AP)

    WB analysis using 20178-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20178-1-AP (EPN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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EPN3 Polyclonal antibody

EPN3 Polyclonal Antibody for WB, ELISA
Cat No. 20178-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Epsin-3, EPS-15-interacting protein 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-10A cells, MCF-7 cells, mouse stomach tissue, rat stomach tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20178-1-AP targets EPN3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPN3 fusion protein Ag14021 相同性解析による交差性が予測される生物種
Full Name epsin 3
Calculated molecular weight 632 aa, 68 kDa
Observed molecular weight68-70 kDa
GenBank accession numberBC001038
Gene Symbol EPN3
Gene ID (NCBI) 55040
RRIDAB_3669327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H201
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Epsin 3 (EPN3) belongs to the epsin family, which also includes EPN1 and EPN2. They belong to the endocytic clathrin family and are involved in receptor endocytosis. EPN3 is expressed in breast cancer and is an oncogene associated with breast cancer prognosis and treatment (PMID: 34779498;PMID: 32541686).

Protocols

Product Specific Protocols
WB protocol for EPN3 antibody 20178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols