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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EPO Monoclonal antibody, PBS Only

EPO Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11F3

Cat no : 66975-1-PBS

Synonyms

EP, EPO, Epoetin, erythropoietin, MVCD2

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using EPO Monoclonal antibody (66975-1-Ig)

    WB analysis of Recombinant protein using 66975-1-Ig (same clone as 66975-1-PBS)

    50 ng recombinant human EPO (cat.NO. HZ-1168) was subjected to SDS-PAGE under reducing condition followed by western blot with 66975-1-Ig (EPO antibody) at dilution of 1:10000. This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using EPO Monoclonal antibody (66975-1-Ig)

    IHC staining of human liver using 66975-1-Ig (same clone as 66975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66975-1-Ig (EPO antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using EPO Monoclonal antibody (66975-1-Ig)

    IHC staining of human liver using 66975-1-Ig (same clone as 66975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66975-1-Ig (EPO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using EPO Monoclonal antibody (66975-1-Ig)

    IHC staining of mouse kidney using 66975-1-Ig (same clone as 66975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66975-1-Ig (EPO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using EPO Monoclonal antibody (66975-1-Ig)

    IHC staining of mouse kidney using 66975-1-Ig (same clone as 66975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66975-1-Ig (EPO antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using EPO Monoclonal antibody (66975-1-Ig)

    IHC staining of mouse kidney using 66975-1-Ig (same clone as 66975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66975-1-Ig (EPO antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using EPO Monoclonal antibody (66975-1-Ig)

    IHC staining of mouse kidney using 66975-1-Ig (same clone as 66975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66975-1-Ig (EPO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66975-1-PBS targets EPO in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EPO fusion protein Ag23054 相同性解析による交差性が予測される生物種
Full Name erythropoietin
Calculated molecular weight 193 aa, 21 kDa
GenBank accession numberBC093628
Gene symbol EPO
Gene ID (NCBI) 2056
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.