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  • Featured Product
  • KD/KO Validated

EPO/Erythropoietin Polyclonal antibody, PBS Only

EPO/Erythropoietin Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17908-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using EPO/Erythropoietin Polyclonal antibody (17908-1-AP)

    WB analysis of HEK-293 using 17908-1-AP (same clone as 17908-1-PBS)

    WB result of EPO/Erythropoietin antibody (17908-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPO transfected HEK-293 cells. This data was developed using the same antibody clone with 17908-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using EPO/Erythropoietin Polyclonal antibody (17908-1-AP)

    IHC staining of rat kidney using 17908-1-AP (same clone as 17908-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17908-1-AP (EPO/Erythropoietin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17908-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using EPO/Erythropoietin Polyclonal antibody (17908-1-AP)

    IHC staining of rat kidney using 17908-1-AP (same clone as 17908-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17908-1-AP (EPO/Erythropoietin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17908-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17908-1-PBS targets EPO/Erythropoietin in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPO/Erythropoietin fusion protein Ag12302 相同性解析による交差性が予測される生物種
Full Name erythropoietin
Calculated molecular weight 193 aa, 21 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC093628
Gene symbol EPO/Erythropoietin
Gene ID (NCBI) 2056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. Erythropoietin (Epo) is a 166 amino acids protein containing three N-glycosylation sites (Asn-24, Asn-38, and Asn-83) and 1 O- glycosylation site (Ser-126) and involved in the regulation of the level of red blood cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.