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  • Featured Product
  • KD/KO Validated

EPS8L2 Polyclonal antibody

EPS8L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20461-1-AP

Synonyms

EPS8 like 2, EPS8 like protein 2, EPS8 related protein 2, EPS8L2, EPS8R2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPS8L2 Polyclonal antibody (20461-1-AP)

    WB analysis using 20461-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20461-1-AP (EPS8L2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using EPS8L2 Polyclonal antibody (20461-1-AP)

    WB analysis of HepG2 using 20461-1-AP

    WB result of EPS8L2 antibody (20461-1-AP; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPS8L2 transfected HepG2 cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using EPS8L2 Polyclonal antibody (20461-1-AP)

    IP experiment of HeLa using 20461-1-AP

    IP result of anti-EPS8L2 (IP:20461-1-AP, 4ug; Detection:20461-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EPS8L2 Polyclonal antibody (20461-1-AP)

    IHC staining of human lung cancer using 20461-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20461-1-AP (EPS8L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPS8L2 Polyclonal antibody (20461-1-AP)

    IF Staining of HeLa using 20461-1-AP

    Immunofluorescent analysis of HeLa cells using 20461-1-AP (EPS8L2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, HepG2 cells, HeLa cells, mouse skin tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20461-1-AP targets EPS8L2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPS8L2 fusion protein Ag14296 相同性解析による交差性が予測される生物種
Full Name EPS8-like 2
Calculated molecular weight 715 aa, 81 kDa
Observed molecular weight 81-85 kDa
GenBank accession numberBC002474
Gene symbol EPS8L2
Gene ID (NCBI) 64787
RRIDAB_10665945
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EPS8L2 (epidermal growth factor receptor pathway substrate 8 L2), a member of the Eps8-like protein family, is a newly identified hair bundle protein that is localized at the tips of stereocilia of both cochlear and vestibular hair cells.

Protocols

Product Specific Protocols
WB protocol for EPS8L2 antibody 20461-1-APDownload protocol
IHC protocol for EPS8L2 antibody 20461-1-APDownload protocol
IF protocol for EPS8L2 antibody 20461-1-APDownload protocol
IP protocol for EPS8L2 antibody 20461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Lab Invest

Nuclear receptor binding factor 2 (NRBF2) is required for learning and memory.

Authors - Xiaosen Ouyang
View Article
mouseIF,WB

Cell Rep

Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease

Authors - Min Yu
View Article