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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EPX Polyclonal antibody

EPX Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, pig, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29755-1-AP

Synonyms

eosinophil peroxidase, EPER, EPO, EPP, EPX, EPX PEN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPX Polyclonal antibody (29755-1-AP)

    WB analysis using 29755-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29755-1-AP (EPX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using EPX Polyclonal antibody (29755-1-AP)

    IHC staining of human hepatocirrhosis using 29755-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29755-1-AP (EPX antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EPX Polyclonal antibody (29755-1-AP)

    IHC staining of human ovary tumor using 29755-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29755-1-AP (EPX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EPX Polyclonal antibody (29755-1-AP)

    IHC staining of human ovary tumor using 29755-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29755-1-AP (EPX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using EPX Polyclonal antibody (29755-1-AP)

    IHC staining of human hepatocirrhosis using 29755-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29755-1-AP (EPX antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inpig bone marrow tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29755-1-AP targets EPX in WB, IHC, ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Reactivity Human, pig, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPX fusion protein Ag30561 相同性解析による交差性が予測される生物種
Full Name eosinophil peroxidase
Calculated molecular weight 81 kDa
Observed molecular weight55 kDa
GenBank accession numberNM_000502
Gene symbol EPX
Gene ID (NCBI) 8288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EPX antibody 29755-1-APDownload protocol
IHC protocol for EPX antibody 29755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols