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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ER Recombinant antibody (84564-4-RR)

    WB analysis using 84564-4-RR (same clone as 84564-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84564-4-RR (ER antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84564-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ER Recombinant antibody (84564-4-RR)

    IHC staining of human ovary cancer using 84564-4-RR (same clone as 84564-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84564-4-RR (ER antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84564-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ER Recombinant antibody (84564-4-RR)

    IF Staining of MCF-7 using 84564-4-RR (same clone as 84564-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ER antibody (84564-4-RR, Clone: 241825D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84564-4-PBS in a different storage buffer formulation.

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ER Recombinant antibody, PBS Only

ER Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84564-4-PBS
Clone No.241825D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ESR1, ESR, ERα, ER-alpha, Era

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84564-4-PBS targets ER in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name estrogen receptor 1
Calculated molecular weight 595 aa, 66 kDa
Observed molecular weight66 kDa
GenBank accession numberBC128573
Gene Symbol ER
Gene ID (NCBI) 2099
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP03372
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]