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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AHSP Polyclonal antibody

AHSP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13643-1-AP

Synonyms

ERAF, Erythroid-associated factor, Erythroid differentiation-related factor, erythroid associated factor, EDRF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AHSP Polyclonal antibody (13643-1-AP)

    WB analysis using 13643-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13643-1-AP (AHSP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using AHSP Polyclonal antibody (13643-1-AP)

    IHC staining of human placenta using 13643-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13643-1-AP (AHSP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using AHSP Polyclonal antibody (13643-1-AP)

    IHC staining of mouse lung using 13643-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13643-1-AP (AHSP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inpig bone marrow tissue
Positive IHC detected inhuman placenta tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13643-1-AP targets AHSP in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHSP fusion protein Ag4588 相同性解析による交差性が予測される生物種
Full Name erythroid associated factor
Calculated molecular weight 102 aa, 12 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC035842
Gene symbol AHSP
Gene ID (NCBI) 51327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AHSP acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development, and specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia (PMID: 12066189).

Protocols

Product Specific Protocols
WB protocol for AHSP antibody 13643-1-APDownload protocol
IHC protocol for AHSP antibody 13643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols