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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using ERAL1 Polyclonal antibody (29465-1-AP)

    WB analysis of HEK-293T using 29465-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 29465-1-AP (ERAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ERAL1 Polyclonal antibody (29465-1-AP)

    WB analysis of K-562 using 29465-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 29465-1-AP (ERAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using ERAL1 Polyclonal antibody (29465-1-AP)

    IHC staining of human colon using 29465-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29465-1-AP (ERAL1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ERAL1 Polyclonal antibody (29465-1-AP)

    IHC staining of human colon using 29465-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29465-1-AP (ERAL1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ERAL1 Polyclonal antibody (29465-1-AP)

    IHC staining of human kidney using 29465-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29465-1-AP (ERAL1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ERAL1 Polyclonal antibody (29465-1-AP)

    IHC staining of human kidney using 29465-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29465-1-AP (ERAL1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ERAL1 Polyclonal antibody

ERAL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29465-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

CEGA, Conserved ERA like GTPase, ERA, Era G protein like 1 (E. coli), ERA W, ERAL1, ERAL1A, hERA, HERA A, HERA B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, K-562 cells
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29465-1-AP targets ERAL1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERAL1 fusion protein Ag30720 相同性解析による交差性が予測される生物種
Full Name Era G-protein-like 1 (E. coli)
Calculated molecular weight 437 aa, 48 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC019094
Gene Symbol ERAL1
Gene ID (NCBI) 26284
RRIDAB_2918311
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75616
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ERAL1, a homologue of Era protein in Escherichia coli, is a member of conserved GTP-binding proteins with RNA-binding activity. ERAL1 localizes to the mitochondrionmitoribosomal subunit, and binds to the 3' terminal stem loop of 12S mitochondrial rRNA and is required for proper assembly of the 28S small mitochondrial ribosomal subunit. Deletion of ERAL1 has been shown to cause mitochondrial dysfunction, growth retardation, and apoptosis.

Protocols

Product Specific Protocols
WB protocol for ERAL1 antibody 29465-1-APDownload protocol
IHC protocol for ERAL1 antibody 29465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols