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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERAP2 Polyclonal antibody

ERAP2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14637-1-AP

Synonyms

ERAP2, L RAP, LRAP

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using ERAP2 Polyclonal antibody (14637-1-AP)

    WB analysis of K-562 using 14637-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14637-1-AP (ERAP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using ERAP2 Polyclonal antibody (14637-1-AP)

    WB analysis of mouse thymus using 14637-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14637-1-AP (ERAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ERAP2 Polyclonal antibody (14637-1-AP)

    WB analysis of A549 using 14637-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14637-1-AP (ERAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ERAP2 Polyclonal antibody (14637-1-AP)

    IHC staining of human lung cancer using 14637-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ERAP2 Polyclonal antibody (14637-1-AP)

    IHC staining of human lung cancer using 14637-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ERAP2 Polyclonal antibody (14637-1-AP)

    IHC staining of human liver using 14637-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ERAP2 Polyclonal antibody (14637-1-AP)

    IHC staining of human liver using 14637-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ERAP2 Polyclonal antibody (14637-1-AP)

    IF Staining of HepG2 using 14637-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inK-562 cells, A549 cells, mouse thymus tissue
Positive IHC detected inhuman lung cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14637-1-AP targets ERAP2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERAP2 fusion protein Ag6216 相同性解析による交差性が予測される生物種
Full Name endoplasmic reticulum aminopeptidase 2
Calculated molecular weight 110 kDa
Observed molecular weight 61-65 kDa
GenBank accession numberBC065240
Gene symbol ERAP2
Gene ID (NCBI) 64167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERAP2(Endoplasmic reticulum aminopeptidase 2) is also named as LRAP(Leukocyte-derived arginine aminopeptidase) and belongs to the peptidase M1 family. It plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. It can form heterodimers with ERAP1(PMID:15908954). It has 4 isoforms produced by alternative splicing. And defects in the expression of this gene may cause improper antigen processing, possibly leading to favor tumor escape from the immune surveillance.