HER2/ErbB2,p185-Specific Recombinant antibody

HER2/ErbB2,p185-Specific Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240802C2

Cat no : 84046-1-RR

Synonyms

ERBB2, HER2, HER 2/neu, HER 2, CD340


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Tested Applications

Positive WB detected inHeLa cells, NCI-H1299 cells, MCF-7 cells, A549 cells, 4T1 cells, SGC-7901 cells
Positive IHC detected inhuman ovarian cancer, mouse uterus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84046-1-RR targets HER2/ErbB2,p185-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Calculated molecular weight 138 kDa
Observed molecular weight185 kDa
GenBank accession numberBC167147
Gene symbol HER2/ErbB2
Gene ID (NCBI) 2064
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.

Protocols

Product Specific Protocols
WB protocol for HER2/ErbB2,p185-Specific antibody 84046-1-RRDownload protocol
IHC protocol for HER2/ErbB2,p185-Specific antibody 84046-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols