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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERBB2IP Monoclonal antibody (68159-1-Ig)

    WB analysis using 68159-1-Ig (same clone as 68159-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68159-1-Ig (ERBB2IP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ERBB2IP Monoclonal antibody (68159-1-Ig)

    WB analysis using 68159-1-Ig (same clone as 68159-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68159-1-Ig (ERBB2IP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ERBB2IP Monoclonal antibody (68159-1-Ig)

    WB analysis of HeLa using 68159-1-Ig (same clone as 68159-1-PBS)

    WB result of ERBB2IP antibody (68159-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERBB2IP transfected HeLa cells. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ERBB2IP Monoclonal antibody (68159-1-Ig)

    IF Staining of MCF-7 using 68159-1-Ig (same clone as 68159-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ERBB2IP antibody (68159-1-Ig, Clone: 1A8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51110-1

    Cytometric bead array standard curve of MP51110-1

    Cytometric bead array standard curve of MP51110-1, ERBB2IP Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68159-1-PBS. Detection antibody: 68159-2-PBS. Standard:Ag18514. Range: 0.195-100 ng/mL.

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  • KD/KO Validated

ERBB2IP Monoclonal antibody, PBS Only (Capture)

ERBB2IP Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68159-1-PBS
Clone No.1A8D4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

LAP2, KIAA1225, ERBIN, Erbb2-interacting protein, Densin-180-like protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68159-1-PBS targets ERBB2IP as part of a matched antibody pair:

MP51110-1: 68159-1-PBS capture and 68159-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERBB2IP fusion protein Ag18514 相同性解析による交差性が予測される生物種
Full Name erbb2 interacting protein
Calculated molecular weight 1412 aa, 158 kDa
Observed molecular weight 150-180 kDa
GenBank accession numberBC115012
Gene Symbol ERBB2IP
Gene ID (NCBI) 55914
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96RT1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ERBB2IP is a cytoplasmic protein that contains leucine-rich repeats (LRR) and PSD95/Dlg1/zo-1 (PDZ) domain (thus named a LAP protein). ERBB2IP interacts, via its single PDZ domain, specifically with ErbB2, but not with ErbB3, ErbB4, or EGFR. It is colocalized with ErbB2 at the basolateral membranes of epithelial cells. In addition, ERBB2IP also interacts with PSD‐95, Lano and with three p120‐related catenins, eBPAG1 and the β4 subunit of the α6β4 integrin, two major components of hemidesmosomes. The molecular weight of ERBB2IP was predicted 150~180 kDa by Western Blot.(PMID: 25288731,PMID: 24252174,PMID: 19289088)