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  • Featured Product
  • KD/KO Validated

ERBB2IP Monoclonal antibody, PBS Only

ERBB2IP Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A8D4

Cat no : 68159-1-PBS

Synonyms

Densin 180 like protein, erbb2 interacting protein, ERBB2IP, ERBIN, KIAA1225, LAP2, Protein LAP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERBB2IP Monoclonal antibody (68159-1-Ig)

    WB analysis using 68159-1-Ig (same clone as 68159-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68159-1-Ig (ERBB2IP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ERBB2IP Monoclonal antibody (68159-1-Ig)

    WB analysis using 68159-1-Ig (same clone as 68159-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68159-1-Ig (ERBB2IP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ERBB2IP Monoclonal antibody (68159-1-Ig)

    WB analysis of HeLa using 68159-1-Ig (same clone as 68159-1-PBS)

    WB result of ERBB2IP antibody (68159-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERBB2IP transfected HeLa cells. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68159-1-PBS targets ERBB2IP in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERBB2IP fusion protein Ag18514 相同性解析による交差性が予測される生物種
Full Name erbb2 interacting protein
Calculated molecular weight 1412 aa, 158 kDa
Observed molecular weight 150-180 kDa
GenBank accession numberBC115012
Gene symbol ERBB2IP
Gene ID (NCBI) 55914
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ERBB2IP is a cytoplasmic protein that contains leucine-rich repeats (LRR) and PSD95/Dlg1/zo-1 (PDZ) domain (thus named a LAP protein). ERBB2IP interacts, via its single PDZ domain, specifically with ErbB2, but not with ErbB3, ErbB4, or EGFR. It is colocalized with ErbB2 at the basolateral membranes of epithelial cells. In addition, ERBB2IP also interacts with PSD‐95, Lano and with three p120‐related catenins, eBPAG1 and the β4 subunit of the α6β4 integrin, two major components of hemidesmosomes. The molecular weight of ERBB2IP was predicted 150~180 kDa by Western Blot.(PMID: 25288731,PMID: 24252174,PMID: 19289088)