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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERBB3 Polyclonal antibody (30452-1-AP)

    WB analysis using 30452-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30452-1-AP (ERBB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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ERBB3 Polyclonal antibody

ERBB3 Polyclonal Antibody for WB, ELISA
Cat No. 30452-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

HER3, erbB3 S, ErbB 3, EC:2.7.10.1, c erbB3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, MDA-MB-453s cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30452-1-AP targets ERBB3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERBB3 fusion protein Ag30713 相同性解析による交差性が予測される生物種
Full Name v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
Observed molecular weight200 kDa
GenBank accession numberBC082992
Gene Symbol ERBB3
Gene ID (NCBI) 2065
RRIDAB_3086320
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21860
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (ErbB-3, HER3) is a member of the EGF receptor tyrosine kinase family including EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3), and ErbB-4 (HER4) that are frequently overexpressed in a variety of carcinomas. These family members form either homodimers or heterodimers upon ligand binding to mediate cell growth. ErbB-3 binds and is activated by neuregulins and NTAK. It forms a heterodimer with each of the other ErbB receptors. ErbB-3 is predominantly expressed in epithelial tissues and the brain and is overexpressed in a subset of human mammary tumors.

Protocols

Product Specific Protocols
WB protocol for ERBB3 antibody 30452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols