ERBB4 Polyclonal antibody
ERBB4 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 19943-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, MCF-7 cells, rat brain tissue |
Positive IHC detected in | mouse lung tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 11 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
Product Information
19943-1-AP targets ERBB4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) |
Calculated molecular weight | 147 kDa |
Observed molecular weight | 180 kDa, 147 kDa |
GenBank accession number | NM_001042599 |
Gene symbol | ERBB4 |
Gene ID (NCBI) | 2066 |
RRID | AB_10646486 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ERBB4, also named p180erbB4 and HER4, belongs to the protein kinase superfamily, Tyr protein kinase family, and EGF receptor subfamily. ERBB4 specifically binds and it is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin, and NTAK. Interaction with these factors induces cell differentiation. ERBB4 is not activated by EGF, TGF-A, and amphiregulin. ERBB4 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody recognizes the C-term of ERBB4. Alternatively spliced variants that encode different protein isoforms with a calculated molecular weight of 144-147 kDa have been described. The apparent molecular weight of full-length ERBB4 is 150-200 kDa due to glycosylation and phosphorylation. Full-length ERBB4 can be cleaved into an 80-kDa ERBB4 intracellular fragment and a 120-kDa extracellular fragment (PMID: 11343971; 16251361).
Protocols
Product Specific Protocols | |
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WB protocol for ERBB4 antibody 19943-1-AP | Download protocol |
IHC protocol for ERBB4 antibody 19943-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) Neuregulin4 Acts on Hypothalamic ErBb4 to Excite Oxytocin Neurons and Preserve Metabolic Homeostasis | ||
J Neuroinflammation EGFR transactivation contributes to neuroinflammation in Streptococcus suis meningitis. | ||
Cell Mol Neurobiol Identifying Genes that Affect Differentiation of Human Neural Stem Cells and Myelination of Mature Oligodendrocytes | ||
DNA Cell Biol AK003290 Protects Myocardial Cells Against Apoptosis and Promotes Cardiac Function Recovery Via miR-539-3p/ErbB4 Axis in Ischemic-Reperfusion Injury. |