ERBB4 Polyclonal antibody

Name: ERBB4 Polyclonal antibody
Brand: Proteintech
SKU: 19943-1-AP

ERBB4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 19943-1-AP

Synonyms

p180erbB4, HER4, ERBB4 intracellular domain, EC:2.7.10.1, E4ICD

Validation Data Gallery

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AllWBIHCIF

WB analysis of mouse brain using 19943-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19943-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19943-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 19943-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 19943-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of mouse lung using 19943-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19943-1-AP (ERBB4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 19943-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 19943-1-AP (ERBB4 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of mouse lung using 19943-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19943-1-AP (ERBB4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 19943-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 19943-1-AP (ERBB4 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of mouse heart using 19943-1-AP
Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (green) and ErbB4 (red, Cat. No 19943-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 488 secondary antibody. ErbB4 is a key member of ErbB signaling and involved in development and was visualized with an Alexa Fluor 555 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB.
×
Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (green) and ErbB4 (red, Cat. No 19943-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 488 secondary antibody. ErbB4 is a key member of ErbB signaling and involved in development and was visualized with an Alexa Fluor 555 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB.

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Cat No. 19943-1-AP

Size/Concentration

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Tested Applications

Positive WB detected in	mouse brain tissue, MCF-7 cells, rat brain tissue
Positive IHC detected in	mouse lung tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 14 publications below
IHC	See 2 publications below
IF	See 2 publications below

Product Information

19943-1-AP targets ERBB4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
Calculated molecular weight	147 kDa
Observed molecular weight	180 kDa, 147 kDa
GenBank accession number	NM_001042599
Gene symbol	ERBB4
Gene ID (NCBI)	2066
RRID	AB_10646486
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ERBB4, also named p180erbB4 and HER4, belongs to the protein kinase superfamily, Tyr protein kinase family, and EGF receptor subfamily. ERBB4 specifically binds and it is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin, and NTAK. Interaction with these factors induces cell differentiation. ERBB4 is not activated by EGF, TGF-A, and amphiregulin. ERBB4 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody recognizes the C-term of ERBB4. Alternatively spliced variants that encode different protein isoforms with a calculated molecular weight of 144-147 kDa have been described. The apparent molecular weight of full-length ERBB4 is 150-200 kDa due to glycosylation and phosphorylation. Full-length ERBB4 can be cleaved into an 80-kDa ERBB4 intracellular fragment and a 120-kDa extracellular fragment (PMID: 11343971; 16251361).

Protocols

Product Specific Protocols
WB protocol for ERBB4 antibody 19943-1-AP	Download protocol
IHC protocol for ERBB4 antibody 19943-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Adv Sci (Weinh) Neuregulin4 Acts on Hypothalamic ErBb4 to Excite Oxytocin Neurons and Preserve Metabolic Homeostasis Authors - Yi Zhang View Article
human	IHC	Respir Res Identification of targetable kinases in idiopathic pulmonary fibrosis. Authors - Hisao Higo View Article
human	WB	Front Oncol DNAJC12 as a Mediator Between ESR1 and ERBB4 in Breast Carcinoma Cells. Authors - Mianjie Lin View Article
pig	WB	J Neuroinflammation EGFR transactivation contributes to neuroinflammation in Streptococcus suis meningitis. Authors - Xiao-Pei Yang View Article
human	WB,IF	Cell Mol Neurobiol Identifying Genes that Affect Differentiation of Human Neural Stem Cells and Myelination of Mature Oligodendrocytes Authors - Dou Ye View Article
mouse	WB	DNA Cell Biol AK003290 Protects Myocardial Cells Against Apoptosis and Promotes Cardiac Function Recovery Via miR-539-3p/ErbB4 Axis in Ischemic-Reperfusion Injury. Authors - Yong Tang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERBB4 Polyclonal antibody

ERBB4 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of mouse brain using 19943-1-AP

WB analysis of mouse brain using 19943-1-AP

WB analysis of mouse brain using 19943-1-AP

WB analysis using 19943-1-AP

WB analysis of MCF-7 using 19943-1-AP

IHC staining of mouse lung using 19943-1-AP

IHC staining of human kidney using 19943-1-AP

IHC staining of mouse lung using 19943-1-AP

IHC staining of human kidney using 19943-1-AP

IF Staining of mouse heart using 19943-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Neuregulin4 Acts on Hypothalamic ErBb4 to Excite Oxytocin Neurons and Preserve Metabolic Homeostasis

Identification of targetable kinases in idiopathic pulmonary fibrosis.

DNAJC12 as a Mediator Between ESR1 and ERBB4 in Breast Carcinoma Cells.

EGFR transactivation contributes to neuroinflammation in Streptococcus suis meningitis.

Identifying Genes that Affect Differentiation of Human Neural Stem Cells and Myelination of Mature Oligodendrocytes

AK003290 Protects Myocardial Cells Against Apoptosis and Promotes Cardiac Function Recovery Via miR-539-3p/ErbB4 Axis in Ischemic-Reperfusion Injury.

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