ERCC5 Monoclonal antibody
ERCC5 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1G2C9
Cat no : 67055-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-29 cells, COLO 320 cells, Daudi cells, Ramos cells, HeLa cells, HepG2 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
67055-1-Ig targets ERCC5 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ERCC5 fusion protein Ag28561 相同性解析による交差性が予測される生物種 |
| Full Name | excision repair cross-complementing rodent repair deficiency, complementation group 5 |
| Calculated molecular weight | 1186 aa, 133 kDa |
| Observed molecular weight | 200 kDa |
| GenBank accession number | BC031522 |
| Gene symbol | ERCC5 |
| Gene ID (NCBI) | 2073 |
| RRID | AB_2882366 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
The human genes correcting DNA repair defects are termed excision-repair cross-complementing or ERCC genes. The ERCC5 gene corrects the excision repair deficiency of Chinese hamster ovary cell line UV135 of complementation group 5. The human ERCC5 gene product is a structure-specific endonuclease required for making the 3-prime incision during DNA nucleotide excision-repair (NER). It also plays an important role in regulating DNA excision repair, removal of bulky lesions caused by environmental chemicals or UV light [PMID:22815677]. The calculated molecular weight of ERCC5 is 133 kDa, but the modified ERCC5 protein is about 200 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ERCC5 antibody 67055-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Pharmacol Triptolide enhances carboplatin-induced apoptosis by inhibiting nucleotide excision repair (NER) activity in melanoma |
