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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using ERCC6L Polyclonal antibody (15688-1-AP)

    WB analysis of human heart using 15688-1-AP (same clone as 15688-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 15688-1-AP (ERCC6L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15688-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ERCC6L Polyclonal antibody (15688-1-AP)

    WB analysis using 15688-1-AP (same clone as 15688-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15688-1-AP (ERCC6L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15688-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ERCC6L Polyclonal antibody (15688-1-AP)

    WB analysis of Jurkat using 15688-1-AP (same clone as 15688-1-PBS)

    WB result of ERCC6L antibody (15688-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERCC6L transfected Jurkat cells. This data was developed using the same antibody clone with 15688-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using ERCC6L Polyclonal antibody (15688-1-AP)

    WB analysis of mouse lung using 15688-1-AP (same clone as 15688-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15688-1-AP (ERCC6L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15688-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ERCC6L Polyclonal antibody (15688-1-AP)

    IHC staining of human colon cancer using 15688-1-AP (same clone as 15688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15688-1-AP (ERCC6L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15688-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ERCC6L Polyclonal antibody (15688-1-AP)

    IHC staining of human colon cancer using 15688-1-AP (same clone as 15688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15688-1-AP (ERCC6L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15688-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ERCC6L Polyclonal antibody, PBS Only

ERCC6L Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15688-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15688-1-PBS targets ERCC6L in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERCC6L fusion protein Ag8223 相同性解析による交差性が予測される生物種
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 6-like
Calculated molecular weight 419 aa, 46 kDa, 141 kDa
Observed molecular weight 180 kDa
GenBank accession numberBC008808
Gene Symbol ERCC6L
Gene ID (NCBI) 54821
RRIDAB_2098171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2NKX8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ERCC6L, also named as Tumor antigen BJ-HCC-15 or PICH, is a 1250 amino acid protein, which contains one helicase ATP-binding domain, one helicase C-terminal domain and two TPR repeats. ERCC6L localizes to kinetochores, inner centromeres and belongs to the SNF2/RAD54 helicase family. ERCC6L as a DNA helicase that acts as an essential component of the spindle assembly checkpoint. The calculated molecular weight of ERCC6L is 140 kDa, but the phosphorylated modification of molecular weight of ERCC6L is about 180kDa.