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- KD/KO Validated
ERGIC1 Polyclonal antibody
ERGIC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 16108-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, mouse liver tissue, L02 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 2 publications below |
Product Information
16108-1-AP targets ERGIC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ERGIC1 fusion protein Ag9025 相同性解析による交差性が予測される生物種 |
| Full Name | endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1 |
| Calculated molecular weight | 290 aa, 33 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC012766 |
| Gene symbol | ERGIC1 |
| Gene ID (NCBI) | 57222 |
| RRID | AB_2098435 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The function of ERGIC1 remains largely unknown. ERGIC1 was highly expressed in most primary prostate tumors. It is an intriguing potential drug target especially for the ERG oncogene expressing tumors (PMID: 22761906). The MW of this protein is 32 kDa, and this antibody specially recognises the 32 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ERGIC1 antibody 16108-1-AP | Download protocol |
| IHC protocol for ERGIC1 antibody 16108-1-AP | Download protocol |
| IF protocol for ERGIC1 antibody 16108-1-AP | Download protocol |
| IP protocol for ERGIC1 antibody 16108-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Sequencing of captive target transcripts identifies the network of regulated genes and functions of primate-specific miR-522. | ||
Nat Commun Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2 | ||
Cell Rep E3 ligase Trim35 inhibits LSD1 demethylase activity through K63-linked ubiquitination and enhances anti-tumor immunity in NSCLC
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