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  • Featured Product
  • KD/KO Validated

ERGIC2 Polyclonal antibody

ERGIC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11927-1-AP

Synonyms

PTX1, ERV41, ERGIC and golgi 2, Endoplasmic reticulum-Golgi intermediate compartment protein 2, CDA14

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ERGIC2 Polyclonal antibody (11927-1-AP)

    WB analysis of HeLa using 11927-1-AP

    WB result of ERGIC2 antibody (11927-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC2 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using ERGIC2 Polyclonal antibody (11927-1-AP)

    WB analysis using 11927-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11927-1-AP (ERGIC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ERGIC2 Polyclonal antibody (11927-1-AP)

    IP experiment of HepG2 using 11927-1-AP

    IP result of anti-ERGIC2 (IP:11927-1-AP, 4ug; Detection:11927-1-AP 1:1000) with HepG2 cells lysate 2900ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ERGIC2 Polyclonal antibody (11927-1-AP)

    IHC staining of human prostate cancer using 11927-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11927-1-AP (ERGIC2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ERGIC2 Polyclonal antibody (11927-1-AP)

    IF Staining of HepG2 using 11927-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11927-1-AP (ERGIC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11927-1-AP targets ERGIC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERGIC2 fusion protein Ag2603 相同性解析による交差性が予測される生物種
Full Name ERGIC and golgi 2
Calculated molecular weight 377 aa, 43 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC000887
Gene symbol ERGIC2
Gene ID (NCBI) 51290
RRIDAB_2098440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERGIC2, also named as Endoplasmic reticulum-Golgi intermediate compartment protein 2 or PTX1, is a 377 amino acid protein, which belongs to the ERGIC family. ERGIC2 is ubiquitously expressed in many tissues. ERGIC2 localizes in the endoplasmic reticulum-Golgi intermediate compartment membrane and may play a role in transport between endoplasmic reticulum and Golgi. ERGIC2 is involved in protein transportation, and participated in the elongation factor 1 alpha regulated mechanisms.

Protocols

Product Specific Protocols
WB protocol for ERGIC2 antibody 11927-1-APDownload protocol
IHC protocol for ERGIC2 antibody 11927-1-APDownload protocol
IF protocol for ERGIC2 antibody 11927-1-APDownload protocol
IP protocol for ERGIC2 antibody 11927-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

DNA Cell Biol

Characterization of a Variant of ERGIC2 Transcript.

Authors - Kwok Simon C M SC
View Article