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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERGIC3 Monoclonal antibody (68398-1-Ig)

    WB analysis using 68398-1-Ig (same clone as 68398-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68398-1-Ig (ERGIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68398-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ERGIC3 Monoclonal antibody (68398-1-Ig)

    WB analysis of HepG2 using 68398-1-Ig (same clone as 68398-1-PBS)

    WB result of ERGIC3 antibody (68398-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC3 transfected HepG2 cells. This data was developed using the same antibody clone with 68398-1-PBS in a different storage buffer formulation.

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ERGIC3 Monoclonal antibody, PBS Only

ERGIC3 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68398-1-PBS
Clone No.1B9F11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

ERV46, Endoplasmic reticulum-Golgi intermediate compartment protein 3, CGI-54, CGI 54, C20orf47

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68398-1-PBS targets ERGIC3 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERGIC3 fusion protein Ag9190 相同性解析による交差性が予測される生物種
Full Name ERGIC and golgi 3
Calculated molecular weight 383 aa, 43 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC009765
Gene Symbol ERGIC3
Gene ID (NCBI) 51614
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y282
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ERGIC3 (Endoplasmic reticulum-Golgi intermediate compartment protein 3 ) is located in the cis face of the Golgi apparatus and vesicular tubular structures between the transitional endoplasmic reticulum (ER) and cis-Golgi. ERGIC3 significantly affects cell growth and causes ER stress-induced cell death, and is involved in the invasion and metastasis in hepatocellular carcinomas (HCC). (PMID: 26177443, PMID: 31142615)