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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERI1 Polyclonal antibody

ERI1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14592-1-AP

Synonyms

HEXO, Eri-1 homolog, ERI 1, EC:3.1.-.-, 3'EXO

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using ERI1 Polyclonal antibody (14592-1-AP)

    WB analysis of K-562 using 14592-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14592-1-AP (ERI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using ERI1 Polyclonal antibody (14592-1-AP)

    WB analysis of HL-60 using 14592-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14592-1-AP (ERI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ERI1 Polyclonal antibody (14592-1-AP)

    WB analysis of HepG2 using 14592-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14592-1-AP (ERI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERI1 Polyclonal antibody (14592-1-AP)

    IHC staining of human liver cancer using 14592-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14592-1-AP (ERI1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ERI1 Polyclonal antibody (14592-1-AP)

    IHC staining of human testis using 14592-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14592-1-AP (ERI1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ERI1 Polyclonal antibody (14592-1-AP)

    IHC staining of human testis using 14592-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14592-1-AP (ERI1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERI1 Polyclonal antibody (14592-1-AP)

    IHC staining of human liver cancer using 14592-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14592-1-AP (ERI1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ERI1 Polyclonal antibody (14592-1-AP)

    IF Staining of HepG2 using 14592-1-AP

    Immunofluorescent analysis of HepG2 cells using 14592-1-AP (ERI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inK-562 cells, HepG2 cells, HL-60 cells
Positive IHC detected inhuman liver cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14592-1-AP targets ERI1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERI1 fusion protein Ag6125 相同性解析による交差性が予測される生物種
Full Name exoribonuclease 1
Calculated molecular weight 40 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC035279
Gene symbol ERI1
Gene ID (NCBI) 90459
RRIDAB_11182393
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERI1, also named as HEXO, 3'HEXO and THEX1, contains one exonuclease domain and one SAP domain. The SAP domain is necessary for binding to the stem-loop structure of histone mRNAs and to form the ternary complex with SLBP, but not for 3'-end histone mRNA exonuclease activity. It is a RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. It is able to bind other mRNAs. ERI1 is required for 5.8S rRNA 3'-end processing. It also binds to 5.8s ribosomal RNA. ERI1 binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.

Protocols

Product Specific Protocols
WB protocol for ERI1 antibody 14592-1-APDownload protocol
IHC protocol for ERI1 antibody 14592-1-APDownload protocol
IF protocol for ERI1 antibody 14592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols