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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERK1/2 Polyclonal antibody, PBS Only

ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 51068-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERK1/2 Polyclonal antibody (51068-1-AP)

    WB analysis using 51068-1-AP (same clone as 51068-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51068-1-AP (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ERK1/2 Polyclonal antibody (51068-1-AP)

    WB analysis using 51068-1-AP (same clone as 51068-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51068-1-AP (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ERK1/2 Polyclonal antibody (51068-1-AP)

    IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51068-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ERK1/2 Polyclonal antibody (51068-1-AP)

    IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51068-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ERK1/2 Polyclonal antibody (51068-1-AP)

    IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51068-1-AP (ERK1/2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ERK1/2 Polyclonal antibody (51068-1-AP)

    IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51068-1-AP (ERK1/2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ERK1/2 Polyclonal antibody (51068-1-AP)

    IF Staining of HeLa using 51068-1-AP (same clone as 51068-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ERK1/2 antibody (51068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ERK1/2 Polyclonal antibody (51068-1-AP)

    IF Staining of HeLa using 51068-1-AP (same clone as 51068-1-PBS)

    Immunofluorescent analysis of HeLa cells using 51068-1-AP (ERK1/2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

51068-1-PBS targets ERK1/2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 1
Calculated molecular weight 360 aa, 41 kDa
Observed molecular weight 38-44 kDa
GenBank accession numberBC017832
Gene symbol ERK2
Gene ID (NCBI) 5594
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.