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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERK1/2 Monoclonal antibody (66192-1-Ig)

    WB analysis using 66192-1-Ig (same clone as 66192-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ERK1/2 Monoclonal antibody (66192-1-Ig)

    WB analysis of HepG2 using 66192-1-Ig (same clone as 66192-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ERK1/2 Monoclonal antibody (66192-1-Ig)

    WB analysis of MCF-7 using 66192-1-Ig (same clone as 66192-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ERK1/2 Monoclonal antibody (66192-1-Ig)

    WB analysis of HeLa using 66192-1-Ig (same clone as 66192-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ERK1/2 Monoclonal antibody (66192-1-Ig)

    IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ERK1/2 Monoclonal antibody (66192-1-Ig)

    IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ERK1/2 Monoclonal antibody (66192-1-Ig)

    IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ERK1/2 Monoclonal antibody (66192-1-Ig)

    IHC staining of human cervical cancer using 66192-1-Ig (same clone as 66192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ERK1/2 Monoclonal antibody (66192-1-Ig)

    IHC staining of human cervical cancer using 66192-1-Ig (same clone as 66192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ERK1/2 Monoclonal antibody (66192-1-Ig)

    IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ERK1/2 Monoclonal antibody (66192-1-Ig)

    IF Staining of HeLa using 66192-1-Ig (same clone as 66192-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ERK1/2 antibody (66192-1-Ig, Clone: 4G8A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

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ERK1/2 Monoclonal antibody, PBS Only

ERK1/2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66192-1-PBS
Clone No.4G8A11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ERK1, ERK, ERK2, ERK-1, EC:2.7.11.24

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66192-1-PBS targets ERK1/2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERK1/2 fusion protein Ag20350 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 3
Calculated molecular weight 43 kDa
Observed molecular weight 38-44 kDa
GenBank accession numberBC013992
Gene Symbol ERK1
Gene ID (NCBI) 5595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27361
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.