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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERK1/2 Recombinant antibody

ERK1/2 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4P8

Cat no : 83533-1-RR

Synonyms

ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERK1/2 Recombinant antibody (83533-1-RR)

    WB analysis using 83533-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83533-1-RR (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ERK1/2 Recombinant antibody (83533-1-RR)

    IF Staining of HeLa using 83533-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ERK1/2 antibody (83533-1-RR, Clone: 4P8 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity experiments of 83533-1-RR

    Affinity experiments of 83533-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83533-1-RR against Human ERK1/2 were performed. The affinity constant is 15.1 nM.

Tested Applications

Positive WB detected inMCF-7 cells, Jurkat cells, HEK-293 cells, HepG2 cells, C6 cells, NIH/3T3 cells, rat brain tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83533-1-RR is ERK1/2 Fusion Protein expressed in E. coli.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ERK1/2 fusion protein Ag20350 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 3
Calculated molecular weight 43 kDa
Observed molecular weight 38-44 kDa
GenBank accession numberBC013992
Gene symbol MAPK3
Gene ID (NCBI) 5595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Protocols

Product Specific Protocols
WB protocol for ERK1/2 antibody 83533-1-RRDownload protocol
IF protocol for ERK1/2 antibody 83533-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols