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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERMN Polyclonal antibody (31432-1-AP)

    WB analysis using 31432-1-AP (same clone as 31432-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31432-1-AP (ERMN antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31432-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ERMN Polyclonal antibody (31432-1-AP)

    IF Staining of mouse brain using 31432-1-AP (same clone as 31432-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ERMN antibody (31432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31432-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using ERMN Polyclonal antibody (31432-1-AP)

    IF Staining of rat brain using 31432-1-AP (same clone as 31432-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ERMN antibody (31432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31432-1-PBS in a different storage buffer formulation.

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ERMN Polyclonal antibody, PBS Only

ERMN Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 31432-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, Indirect ELISA

Synonyms

KIAA1189, Juxtanodin, JN, Ermin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31432-1-PBS targets ERMN in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERMN fusion protein Ag35648 相同性解析による交差性が予測される生物種
Full Name ermin, ERM-like protein
Observed molecular weight 45 kDa
GenBank accession numberBC026345
Gene Symbol ERMN
Gene ID (NCBI) 57471
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8TAM6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ERMN is associated with cytoskeletal rearrangements and the stability of myelin sheath. A study has shown that ERMN is expressed specifically in oligodendrocytes in the central nervous system (CNS), and is involved in the formation of myelin (PMID: 20934411). The predicted MW of ERMN is 33 KDa, while western blot analyses detected a 45-50 kDa single band protein as a result of phosphorylation modification.