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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERMN Monoclonal antibody (66605-1-Ig)

    WB analysis using 66605-1-Ig (same clone as 66605-1-PBS)

    Various tissues were subjected to SDS PAGE followed by western blot with 66605-1-Ig (ERMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66605-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ERMN Monoclonal antibody (66605-1-Ig)

    IHC staining of mouse brain using 66605-1-Ig (same clone as 66605-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66605-1-Ig (ERMN antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66605-1-PBS in a different storage buffer formulation.

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ERMN Monoclonal antibody, PBS Only

ERMN Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66605-1-PBS
Clone No.2B1C1

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

ermin, ermin, ERM like protein, ERMN, JN, Juxtanodin, KIAA1189

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66605-1-PBS targets ERMN in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ERMN fusion protein Ag27040 相同性解析による交差性が予測される生物種
Full Name ermin, ERM-like protein
Observed molecular weight 50 kDa
GenBank accession numberBC026345
Gene Symbol ERMN
Gene ID (NCBI) 57471
RRIDAB_2881965
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TAM6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ERMN is associated with cytoskeletal rearrangements and the stability of myelin sheath. A study has shown that ERMN is expressed specifically in oligodendrocytes in the central nervous system (CNS), and is involved in the formation of myelin (PMID: 20934411). The predicted MW of ERMN is 33 KDa, while western blot analyses of different tissue lysates using 66605-1-Ig antibody to ERMN detected a 50 kDa single band protein as a result of phosphorylation modification.