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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERO1L Polyclonal antibody, PBS Only

ERO1L Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12007-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse ovary tissue using ERO1L Polyclonal antibody (12007-1-AP)

    WB analysis of mouse ovary using 12007-1-AP (same clone as 12007-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12007-1-AP (ERO1L antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12007-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using ERO1L Polyclonal antibody (12007-1-AP)

    WB analysis of A431 using 12007-1-AP (same clone as 12007-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 12007-1-AP (ERO1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12007-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ERO1L Polyclonal antibody (12007-1-AP)

    IHC staining of human pancreas cancer using 12007-1-AP (same clone as 12007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12007-1-AP (ERO1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12007-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ERO1L Polyclonal antibody (12007-1-AP)

    IHC staining of human pancreas cancer using 12007-1-AP (same clone as 12007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12007-1-AP (ERO1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12007-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ERO1L Polyclonal antibody (12007-1-AP)

    IHC staining of human pancreas cancer using 12007-1-AP (same clone as 12007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12007-1-AP (ERO1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12007-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ERO1L Polyclonal antibody (12007-1-AP)

    IHC staining of human pancreas cancer using 12007-1-AP (same clone as 12007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12007-1-AP (ERO1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12007-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12007-1-PBS targets ERO1L in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERO1L fusion protein Ag2620 相同性解析による交差性が予測される生物種
Full Name ERO1-like (S. cerevisiae)
Calculated molecular weight 468 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC008674
Gene symbol ERO1L
Gene ID (NCBI) 30001
RRIDAB_10666441
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ERO1L, also named as ERO1-alpha, is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. It is required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. This antibody has no cross reaction to ERO1.