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  • Featured Product
  • KD/KO Validated

ERO1L Monoclonal antibody, PBS Only (Capture)

ERO1L Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G12E11

Cat no : 67416-1-PBS

Synonyms

ERO1 L, ERO1 alpha, Endoplasmic reticulum oxidoreductase alpha, Endoplasmic oxidoreductin-1-like protein, EC:1.8.4.-

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ERO1L Monoclonal antibody (67416-1-Ig)

    WB analysis of HepG2 using 67416-1-Ig (same clone as 67416-1-PBS)

    WB result of ERO1L antibody (67416-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERO1L transfected HepG2 cells. This data was developed using the same antibody clone with 67416-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ERO1L Monoclonal antibody (67416-1-Ig)

    WB analysis using 67416-1-Ig (same clone as 67416-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67416-1-Ig (ERO1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ERO1L Monoclonal antibody (67416-1-Ig)

    IHC staining of human pancreas cancer using 67416-1-Ig (same clone as 67416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67416-1-Ig (ERO1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ERO1L Monoclonal antibody (67416-1-Ig)

    IHC staining of human pancreas cancer using 67416-1-Ig (same clone as 67416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67416-1-Ig (ERO1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ERO1L Monoclonal antibody (67416-1-Ig)

    IHC staining of human stomach cancer using 67416-1-Ig (same clone as 67416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67416-1-Ig (ERO1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ERO1L Monoclonal antibody (67416-1-Ig)

    IHC staining of human stomach cancer using 67416-1-Ig (same clone as 67416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67416-1-Ig (ERO1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67416-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human stomach cancer tissue using ERO1L Monoclonal antibody (67416-1-Ig)

    IF Staining of human stomach cancer using 67416-1-Ig (same clone as 67416-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using ERO1L antibody (67416-1-Ig, Clone: 1G12E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67416-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ERO1L Monoclonal antibody (67416-1-Ig)

    IF Staining of HEK-293 using 67416-1-Ig (same clone as 67416-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ERO1L antibody (67416-1-Ig, Clone: 1G12E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67416-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51017-4

    Cytometric bead array standard curve of MP51017-4

    Cytometric bead array standard curve of MP51017-4, ERO1L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67416-1-PBS. Detection antibody: 67416-5-PBS. Standard:Ag29910. Range: 3.125-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67416-1-PBS targets ERO1L as part of a matched antibody pair:

MP51017-4: 67416-1-PBS capture and 67416-5-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ERO1L fusion protein Ag29910 相同性解析による交差性が予測される生物種
Full Name ERO1-like (S. cerevisiae)
Calculated molecular weight 468 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC008674
Gene symbol ERO1L
Gene ID (NCBI) 30001
RRIDAB_2882656
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ERO1L, also named as ERO1-alpha, is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. It is required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. ERO1L has a calculated molecular weight of 54 kDa and can be detected as 60kDa.