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- KD/KO Validated
ERO1LB Polyclonal antibody
ERO1LB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 11261-2-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, human placenta tissue |
| Positive IHC detected in | human pancreas cancer tissue, human oesophagus tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
Product Information
11261-2-AP targets ERO1LB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ERO1LB fusion protein Ag1776 相同性解析による交差性が予測される生物種 |
| Full Name | ERO1-like beta (S. cerevisiae) |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 40~50 kDa |
| GenBank accession number | BC032823 |
| Gene symbol | ERO1LB |
| Gene ID (NCBI) | 56605 |
| RRID | AB_2101503 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Endoplasmic reticulum oxidoreductases (Eros) are essential for the formation of disulfide bonds. Mammals express two related Ero proteins, Ero1L alpha and Ero1L beta. The two homologous proteins can form homodimers and mixed heterodimers. ERO1-Lα and -β are highly expressed in organs with a high demand for secretion, consistent with their roles in oxidative folding of ER proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ERO1LB antibody 11261-2-AP | Download protocol |
| IHC protocol for ERO1LB antibody 11261-2-AP | Download protocol |
| IF protocol for ERO1LB antibody 11261-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Curcumin Facilitates a Transitory Cellular Stress Response in Trembler-J Mice. | ||
J Biol Chem Action of protein disulfide isomerase on proinsulin exit from endoplasmic reticulum of pancreatic β-cells. | ||
Sci Rep Hypoxia-inducible ERO1α promotes cancer progression through modulation of integrin-β1 modification and signalling in HCT116 colorectal cancer cells.
| ||
J Toxicol Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease. | ||
ACS Bio Med Chem Au Identification of Natural Product Sulfuretin Derivatives as Inhibitors for the Endoplasmic Reticulum Redox Protein ERO1α
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