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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERP44 Monoclonal antibody

ERP44 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A2C1

Cat no : 67426-1-Ig

Synonyms

ER protein 44, ERP44, KIAA0573, PDIA10, TXNDC4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERP44 Monoclonal antibody (67426-1-Ig)

    WB analysis using 67426-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67426-1-Ig (ERP44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ERP44 Monoclonal antibody (67426-1-Ig)

    WB analysis using 67426-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67426-1-Ig (ERP44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ERP44 Monoclonal antibody (67426-1-Ig)

    IHC staining of human breast cancer using 67426-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67426-1-Ig (ERP44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ERP44 Monoclonal antibody (67426-1-Ig)

    IHC staining of human breast cancer using 67426-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67426-1-Ig (ERP44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERP44 Monoclonal antibody (67426-1-Ig)

    IHC staining of human liver cancer using 67426-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67426-1-Ig (ERP44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERP44 Monoclonal antibody (67426-1-Ig)

    IHC staining of human liver cancer using 67426-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67426-1-Ig (ERP44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67426-1-Ig targets ERP44 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERP44 fusion protein Ag8818 相同性解析による交差性が予測される生物種
Full Name endoplasmic reticulum protein 44
Calculated molecular weight 406 aa, 47 kDa
Observed molecular weight 44-47 kDa
GenBank accession numberBC005374
Gene symbol ERP44
Gene ID (NCBI) 23071
RRIDAB_2882665
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

TXNDC4 is also named as KIAA0573, ERP44, PDIA10, ERp44 and belongs to the thioredoxin family (TRX). It may be involved in electron transport, protein folding, response to unfolded protein, glycoprotein metabolism, secretory pathway. It may have a role in the control of oxidative protein folding in the endoplasmic reticulum.

Protocols

Product Specific Protocols
WB protocol for ERP44 antibody 67426-1-IgDownload protocol
IHC protocol for ERP44 antibody 67426-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols