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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERp72 Monoclonal antibody, PBS Only

ERp72 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D5F3

Cat no : 66365-1-PBS

Synonyms

Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERp72, PDIA4, Protein disulfide isomerase A4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERp72 Monoclonal antibody (66365-1-Ig)

    WB analysis using 66365-1-Ig (same clone as 66365-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66365-1-Ig (ERp72 antibody) at dilution of 1:4900 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERp72 Monoclonal antibody (66365-1-Ig)

    IHC staining of human liver cancer using 66365-1-Ig (same clone as 66365-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66365-1-Ig (ERp72 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERp72 Monoclonal antibody (66365-1-Ig)

    IHC staining of human liver cancer using 66365-1-Ig (same clone as 66365-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66365-1-Ig (ERp72 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66365-1-PBS targets ERp72 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERp72 fusion protein Ag6873 相同性解析による交差性が予測される生物種
Full Name protein disulfide isomerase family A, member 4
Calculated molecular weight 73 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC000425
Gene symbol ERp72
Gene ID (NCBI) 9601
RRIDAB_2881745
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PDIA4 (Protein disulfide-isomerase A4) is also named ERP70, ERP72, and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full-length protein has a signal peptide with 20 amino acids.