Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, mouse testis, rat testis |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
23525-1-AP targets ESCO2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ESCO2 fusion protein Ag18893 相同性解析による交差性が予測される生物種 |
| Full Name | establishment of cohesion 1 homolog 2 (S. cerevisiae) |
| Calculated molecular weight | 601 aa, 68 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC146562 |
| Gene Symbol | ESCO2 |
| Gene ID (NCBI) | 157570 |
| RRID | AB_2879292 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q56NI9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ESCO2 is also known as Establishment of Sister Chromatid Cohesion N-Acetyltransferase 2, EFO2. This gene encodes a member of the family of acetyltransferases involved in the establishment of sister chromatid cohesion during S phase and postreplicative sister chromatid cohesion induced by double-strand breaks (PubMed: 15821733). Mutations in the ESCO2 gene are associated with a rare genetic disorder called Roberts syndrome or SC phocomelia syndrome (PubMed: 16380922). The loss of function or impaired activity of the ESCO2 protein due to mutations disrupts the proper cohesion of sister chromatids, leading to chromosomal abnormalities and the characteristic features of Roberts syndrome. ESCO2 can be detected as about 65-70 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ESCO2 antibody 23525-1-AP | Download protocol |
| IHC protocol for ESCO2 antibody 23525-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Kaohsiung J Med Sci Long noncoding RNA ZFPM2-AS1 regulates renal cell carcinoma progression via miR-130a-3p/ESCO2. | ||
BMC Cancer ESCO2's oncogenic role in human tumors: a pan-cancer analysis and experimental validation | ||
Proc Natl Acad Sci U S A Lactylation of RNA m6A demethylase ALKBH5 promotes innate immune response to DNA herpesviruses and mpox virus | ||
J Dairy Sci Identification of Candidate Genes Related to Hybrid Sterility by Genomic Structural Variation and Transcriptome Analyses in Cattle-yak | ||