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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using ESD Polyclonal antibody (15244-1-AP)

    WB analysis of K-562 using 15244-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15244-1-AP (ESD antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HAP1 cells using ESD Polyclonal antibody (15244-1-AP)

    WB analysis of HAP1 using 15244-1-AP

    WB result of ESD antibody (15244-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and ESD knockout HAP1 cells.

  • Western Blot (WB) analysis of HeLa cells using ESD Polyclonal antibody (15244-1-AP)

    WB analysis of HeLa using 15244-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15244-1-AP (ESD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ESD Polyclonal antibody (15244-1-AP)

    WB analysis of K-562 using 15244-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15244-1-AP (ESD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

ESD Polyclonal antibody

ESD Polyclonal Antibody for WB, ELISA
Cat No. 15244-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

S-formylglutathione hydrolase, Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HAP1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15244-1-AP targets ESD in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ESD fusion protein Ag7457 相同性解析による交差性が予測される生物種
Full Name esterase D/formylglutathione hydrolase
Calculated molecular weight 31 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC001169
Gene Symbol ESD
Gene ID (NCBI) 2098
RRIDAB_2101686
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10768
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Esterase D (ESD) is a non-specific esterase widely distributed in various organisms and is also named S-Formylglutathione Hydrolase (SFGH). ESD is a member of the carboxylesterase family and has both carboxylesterase and thioesterase activities. ESD plays an important role in the process of glutathione-dependent detoxification, regulating cholesterol efflux and virus infection in humans, and is closely related to the development of tumors. ESD as a Genetic Marker for Retinoblastoma (PMID: 32247735, PMID: 34875997, PMID: 35627173). The calculated molecular weight of ESD is 31 kDa.

Protocols

Product Specific Protocols
WB protocol for ESD antibody 15244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols