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  • Featured Product
  • KD/KO Validated

ESD Monoclonal antibody

ESD Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F9A7

Cat no : 67069-1-Ig

Synonyms

Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12, EC:3.1.1.56

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ESD Monoclonal antibody (67069-1-Ig)

    WB analysis using 67069-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67069-1-Ig (ESD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ESD Monoclonal antibody (67069-1-Ig)

    WB analysis of HeLa using 67069-1-Ig

    WB result of ESD antibody (67069-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ESD transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using ESD Monoclonal antibody (67069-1-Ig)

    WB analysis using 67069-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67069-1-Ig (ESD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, PC-3 cells, Caco-2 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67069-1-Ig targets ESD in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ESD fusion protein Ag7487 相同性解析による交差性が予測される生物種
Full Name esterase D/formylglutathione hydrolase
Calculated molecular weight 31 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC001169
Gene symbol ESD
Gene ID (NCBI) 2098
RRIDAB_2882378
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ESD antibody 67069-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols