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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ESF1 Polyclonal antibody

ESF1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23496-1-AP

Synonyms

ABT1 associated protein, ABTAP, C20orf6, ESF1, ESF1 homolog, HDCMC28P

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using ESF1 Polyclonal antibody (23496-1-AP)

    WB analysis of mouse heart using 23496-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 23496-1-AP (ESF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ESF1 Polyclonal antibody (23496-1-AP)

    IF Staining of MCF-7 using 23496-1-AP

    Immunofluorescent analysis of MCF-7 cells using 23496-1-AP (ESF1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

Positive WB detected inmouse heart tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23496-1-AP is ESF1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ESF1 fusion protein Ag19037 相同性解析による交差性が予測される生物種
Full Name ESF1, nucleolar pre-rRNA processing protein, homolog (S. cerevisiae)
Calculated molecular weight 851 aa, 99 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC146542
Gene symbol ESF1
Gene ID (NCBI) 51575
RRIDAB_2879287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ESF1 antibody 23496-1-APDownload protocol
IF protocol for ESF1 antibody 23496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Clin Proteomics

ESF1 and MIPEP proteins promote estrogen receptor-positive breast cancer proliferation and are associated with patient prognosis

Authors - Qing Yu
View Article
humanIF

bioRxiv

Mapping and engineering RNA-controlled architecture of the multiphase nucleolus

Authors - S A Quinodoz
View Article